What is NAD+?

Overview

NAD+ isn’t a supplement in the casual sense — it’s the cofactor that makes 500+ enzymes work. CD38 degrades it. Sirtuins consume it. PARPs burn it during DNA repair. By age 50, tissue NAD+ is down 40–60% and the cascade starts showing up as metabolic drift, DNA-damage accumulation, and declining mitochondrial capacity. Why direct NAD+ matters: precursor pathways (NR, NMN) rely on intact salvage enzymes. In aged or metabolically compromised tissue those enzymes themselves are downregulated. Direct NAD+ bypasses the bottleneck. IF the goal is sirtuin-1 activation and PARP-mediated DNA repair, THEN NAD+ is the rate-limiting cofactor. SO any longevity-adjacent protocol needs NAD+ measured, not assumed. Independently verified at ≥99% purity, batch-tested, CoA included with every vial.

Research areas

• Sirtuin activation

  <p>SIRT1, SIRT3, and SIRT6 are NAD+-dependent deacetylases tied to metabolic gene regulation, mitochondrial biogenesis, and genomic stability. NAD+ availability is the rate-limiting factor for all three.</p>

• DNA repair capacity

  <p>PARP1 consumes NAD+ as its substrate during base-excision and single-strand break repair. Cells under genotoxic stress deplete local NAD+ pools rapidly; exogenous supplementation sustains repair throughput in published in-vitro models.</p>

• Mitochondrial output

  <p>Electron-transport chain complexes I and III require NAD+/NADH cycling. Aged muscle tissue shows restored oxygen consumption and ATP output when NAD+ is restored in animal models.</p>

• Delivery considerations

  <p>Lyophilized NAD+ reconstitutes in bacteriostatic water. Subcutaneous and IV protocols are the most frequently studied routes. Sensitive to light and heat — refrigerate reconstituted solution and use within 30 days.</p>

Storage & handling

Lyophilized NAD+is stored at –20 °C protected from light. Once reconstituted with bacteriostatic water, store at 2–8 °C and use within 30 days for maximum stability.

Frequently asked questions

What is NAD+?

NAD+ (NAD+) is a research compound studied in the area of longevity & anti-aging. The coenzyme behind sirtuin activity, DNA repair, and mitochondrial output. Tissue levels collapse with age — direct NAD+ bypasses the salvage bottleneck.

What is the CAS number of NAD+?

The CAS registry number of NAD+ is 53-84-9.

How should NAD+ be stored?

Lyophilized NAD+ should be stored at –20 °C protected from light. Once reconstituted with bacteriostatic water, store at 2–8 °C and use within 30 days for maximum stability.

How is the purity of NAD+ verified?

Every Woosh Labs batch of NAD+ is independently tested by third-party HPLC and mass spectrometry, with a batch-specific Certificate of Analysis published verifying ≥99% purity.

Is NAD+ for human use?

No. NAD+ sold by Woosh Labs is strictly for in-vitro laboratory research by qualified professionals. It is not for human or veterinary use of any kind.